human dlbcl cell lines u-2932  (Procell Inc)

Journal: Cancers

Article Title: Sex- and Female Age-Dependent Differences in Gene Expression in Diffuse Large B-Cell Lymphoma—Possible Estrogen Effects

doi: 10.3390/cancers15041298

Figure Lengend Snippet: Volcano plot showing sex differences in gene expression in DLBCL. Genes were considered to be differentially expressed when adjusted p -value < 0.05. Upregulated genes are marked in red, downregulated genes are marked in green, and not significant genes are marked in black.

Article Snippet: The human ABC DLBCL cell line U2932 used in this study was obtained from DSMZ (Leibniz Institute, Braunschweig, Germany) and cultured as previously described [ ].

Techniques: Gene Expression

Journal: Cancers

Article Title: Sex- and Female Age-Dependent Differences in Gene Expression in Diffuse Large B-Cell Lymphoma—Possible Estrogen Effects

doi: 10.3390/cancers15041298

Figure Lengend Snippet: Sex differences in gene expression and pathways among molecular subtypes of DLBCL. ( A ), Venn diagram showing the distribution of the differentially expressed genes among the DLBCL subtypes (adj. p -value < 0.05). The table shows the number of samples for each subtype and sex. ( B ), Venn diagram depicting the significant pathways showing a sex difference among the DLBCL subtypes as analyzed by GSEA (adj. p -value < 0.05).

Article Snippet: The human ABC DLBCL cell line U2932 used in this study was obtained from DSMZ (Leibniz Institute, Braunschweig, Germany) and cultured as previously described [ ].

Techniques: Gene Expression

Journal: Cancers

Article Title: Sex- and Female Age-Dependent Differences in Gene Expression in Diffuse Large B-Cell Lymphoma—Possible Estrogen Effects

doi: 10.3390/cancers15041298

Figure Lengend Snippet: Venn diagram showing the number of genes that are differentially expressed in pre- vs. postmenopausal females among the ABC and GCB subtypes and the overlap of differentially expressed genes in pre- (≤52 years of age) vs. post (≥65 years of age)-menopausal females in the ABC and GCB DLBCL subtypes (adj. p -value < 0.05). The table shows the number of DLBCL cases in the ABC and GCB subgroups and in pre- and postmenopausal females, respectively. * This is the explanation (the names) of the five genes that overlap between the GCG and ABC subtypes of female pre- vs. postmenopausal patients.

Article Snippet: The human ABC DLBCL cell line U2932 used in this study was obtained from DSMZ (Leibniz Institute, Braunschweig, Germany) and cultured as previously described [ ].

Techniques:

Journal: Cancers

Article Title: Sex- and Female Age-Dependent Differences in Gene Expression in Diffuse Large B-Cell Lymphoma—Possible Estrogen Effects

doi: 10.3390/cancers15041298

Figure Lengend Snippet: Identification of genes that likely are regulated by estrogens in the ABC and GCB DLBCL subtypes, respectively. ( A ), Venn diagram showing the overlap of differentially expressed genes in the ABC DLBCL subtype. ( B ), Bar graph showing the log2-fold change of genes that are likely regulated by estrogen in the ABC subtype. Upregulated genes are marked in red, and downregulated genes are marked in blue. ( C ), Venn diagram showing the overlap of differentially expressed genes in the GCB DLBCL subtype. ( D ), Bar graph showing the log2-fold change of genes that are likely regulated by estrogens in the GCB subtype. Upregulated genes are marked in red, and downregulated genes are marked in blue.

Article Snippet: The human ABC DLBCL cell line U2932 used in this study was obtained from DSMZ (Leibniz Institute, Braunschweig, Germany) and cultured as previously described [ ].

Techniques:

Journal: Cancers

Article Title: Sex- and Female Age-Dependent Differences in Gene Expression in Diffuse Large B-Cell Lymphoma—Possible Estrogen Effects

doi: 10.3390/cancers15041298

Figure Lengend Snippet: Representative immunohistochemistry staining of DLBCL lymphoma tissue for NR4A2 ( A ) and MUC5B ( B ). Immunohistochemical staining of DLBCL for NR4A2 ( A ) and MUC5B ( B ). Representative staining of samples with low ( left ) and high ( right ) expression, respectively. Black arrows mark examples of expression of NR4A2 and MUC5B in tumor cells, while blue arrows mark expression in non-malignant cells.

Article Snippet: The human ABC DLBCL cell line U2932 used in this study was obtained from DSMZ (Leibniz Institute, Braunschweig, Germany) and cultured as previously described [ ].

Techniques: Immunohistochemistry, Staining, Immunohistochemical staining, Expressing

Journal: Cancers

Article Title: Sex- and Female Age-Dependent Differences in Gene Expression in Diffuse Large B-Cell Lymphoma—Possible Estrogen Effects

doi: 10.3390/cancers15041298

Figure Lengend Snippet: Correlation between NR4A2 expression and overall survival among female ABC DLBCL patients. NR4A2 expression data for each individual female ABC DLBCL and individual survival data were obtained from the EGAD00001003600 data set. Optimal cut point for high vs. low NR4A2 expression was determined using maximally selected rank statistics from the “maxstat” R package. The number of individuals with high and low NR4A2 expression after optimal cut point determination was 55 and 77, respectively. The R packages “survival” and “survminer” were used for the survival analysis. The pink and blue areas show lower 95% to upper 95% CI for low and high NR4A2 expression, respectively. p = 0.008 for the difference in survival between the high and low NR4A2 -expressing individuals.

Article Snippet: The human ABC DLBCL cell line U2932 used in this study was obtained from DSMZ (Leibniz Institute, Braunschweig, Germany) and cultured as previously described [ ].

Techniques: Expressing

Journal: Cancers

Article Title: Sex- and Female Age-Dependent Differences in Gene Expression in Diffuse Large B-Cell Lymphoma—Possible Estrogen Effects

doi: 10.3390/cancers15041298

Figure Lengend Snippet: Inhibition of estrogen synthesis by the aromatase inhibitor letrozole stimulates the growth of U2932 tumors and affects the expression of NR4A and MUC5B . ( A ), Male NSG mice were injected subcutaneously with U2932 cells and treated subcutaneously daily with vehicle or the aromatase inhibitor letrozole (10 μg/mouse). The vehicle group consisted of 7 mice, letrozole group consisted of 8 mice. Tumor size was measured daily. ( B ), RT-qPCR analysis of NR4A1 , NR4A2 , NR4A3 and MUC5B . Results are presented as relative expressions (mean ± SD). An unpaired two-tailed t-test was used for statistical analysis between the two groups (* p < 0.05, ** p < 0.01).

Article Snippet: The human ABC DLBCL cell line U2932 used in this study was obtained from DSMZ (Leibniz Institute, Braunschweig, Germany) and cultured as previously described [ ].

Techniques: Inhibition, Expressing, Injection, Quantitative RT-PCR, Two Tailed Test

Journal: Cancers

Article Title: Screening for Prognostic microRNAs Associated with Treatment Failure in Diffuse Large B Cell Lymphoma

doi: 10.3390/cancers14041065

Figure Lengend Snippet: High levels of miR-1244, miR-193b-5p, or miR-1231 were related to worse event-free survival (EFS) and overall survival (OS). The expression of miR-1244 ( A , B ), miR-93b-5p ( C , D ), and miR-1231 ( E , F ) was studied for their impact on EFS and OS, respectively, in our series of patients with diffuse large B-cell lymphoma (DLBCL). EFS and OS were compared using the Kaplan–Meier curves and log rank test between the high (red line) and low (blue line) expression groups determined by each miRNA-specific threshold. p value indicates statistical significance.

Article Snippet: The Human DLBCL cell line U-2932 (ACC 633) was obtained from the DSMZ (Leibniz-Institut DSMZ German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany).

Techniques: Expressing

Journal: Cancers

Article Title: Screening for Prognostic microRNAs Associated with Treatment Failure in Diffuse Large B Cell Lymphoma

doi: 10.3390/cancers14041065

Figure Lengend Snippet: Inhibition, but not expression, of miR-1244, miR-193b-5p or miR-1231 reduced cell viability in human diffuse large B-cell lymphoma (DLBCL) cells. U-2932 cells were reverse transfected separately with 100 nM of miR-1244, miR-193b-5p, or miR-1231 miRNA mimics ( A ) or inhibitors ( B ) for 48 h. In parallel, another set of cells were reverse transfected with miRNA negative control #1 (miR control (−)) or left entirely untreated. Cell viability was determined as detailed in Materials and Methods. Each column represents mean ± SEM of 3 independent experiments performed in triplicate, and normalized to cells transfected with miR control (−) (taken as 100%). * p < 0.05 and ** p < 0.001 versus cells transfected with miR control (−).

Article Snippet: The Human DLBCL cell line U-2932 (ACC 633) was obtained from the DSMZ (Leibniz-Institut DSMZ German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany).

Techniques: Inhibition, Expressing, Transfection, Negative Control, Control

Journal: Cancers

Article Title: Screening for Prognostic microRNAs Associated with Treatment Failure in Diffuse Large B Cell Lymphoma

doi: 10.3390/cancers14041065

Figure Lengend Snippet: Expression of miR-1244 and miR-193b-5p, but not miR-1231, blocked the antitumoral effect of CHOP in human diffuse large B-cell lymphoma (DLBCL) cells. U-2932 cells were reverse transfected separately with 100 nM of miR-1244 ( A ), miR-193b-5p ( B ), or miR-1231 ( C ) mimics or with miRNA negative control #1 (miR control (−)). 24 h after transfection, cells were treated with vehicle (1% DMSO) or with increasing concentrations (0.3, 1, and 3 µg/mL) of CHOP. Cell viability was determined as detailed in Materials and Methods. Each column represents mean ± SEM of 4 independent experiments performed in triplicate, and normalized to cells transfected with miR control (−) (taken as 100%). ** p < 0.01 and *** p < 0.001 versus cells transfected with miR control (−) and treated with vehicle. ## p < 0.01 and ### p < 0.001 versus their respective cells transfected with miR control (−).

Article Snippet: The Human DLBCL cell line U-2932 (ACC 633) was obtained from the DSMZ (Leibniz-Institut DSMZ German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany).

Techniques: Expressing, Transfection, Negative Control, Control